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Real-time PCR Miner


Stanford Reference:

04-261


Abstract


Researchers at Stanford have developed an algorithm that provides an objective and noise-resistant method to analyze qRT-PCR (quantitative real-time polymerase chain reaction) results independent of the specific equipment used to perform PCR reactions.

qRT-PCR has become the method of choice for rapid, sensitive, quantitative comparison of RNA transcript abundance. Useful data from this method depends on fitting data to theoretical curves that enable computation of mRNA levels. Calculating qRT-PCR results requires parameters such as reaction efficiency and the fractional cycle number at threshold (CT) to be used. However many algorithms currently in use only estimate these important parameters. The Real-time PCR Miner software provides an objective method for quantifying qRT-PCR results using calculations based on the kinetics of individual PCR reactions without the need of the standard curve, independent of any assumptions or subjective judgments which allow direct calculation of efficiency and CT.

Applications


  • RNA quantification - this algorithm can be used to quantify RNA transcript level accurately and objectively by rtPCR.

Advantages


  • Accurate - this algorithm fully accounts for both the background noise of the reaction and the need to limit the quantification to the logarithmic phase of the PCR amplification. In addition, it is independent of the assumption that the amplification efficiency for all samples is comparable.
  • Objective - the algorithm is independent of the investigator's choices and does not introduce additional systematic error such as the standard curve method.
  • Platform-independent - the algorithm is based on the information contained in the kinetics of the individual PCR reactions. The noise-resistant property of this algorithm makes it work across various platforms.
  • Inexpensive - no additional enzymes and reagents needed.
  • Fast and convenient -no need to make a standard curve.
  • Broad applications - because this algorithm can provide the detailed information for individual samples, it can be also used to assess quality of the experiment and to identify unexpected variance in amplification.

Publications



Innovators & Portfolio


  • Russell Fernald   
  • Sheng Zhao   

Date Released

 11/2/2012
 

Licensing Contact


Imelda Oropeza, Licensing Associate
650-725-9039 (Business)
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Related Keywords


genomics: bioinformatic   research tool: PCR   quantitative PCR   real-time PCR   quantitative real time   genomics   research tool   
 

   

  

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