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Cell-based assay for Wnt signaling


Stanford Reference:

05-164


Abstract


Cells that stably express a luciferase reporter construct, driven by the TCF transcription factor have been made. These cells respond to added Wnt protein in a highly reproducible and quantifiable way and are very suitable to test for the activity of candidate inhibitors of Wnt signaling, such as small molecules.

The cells are transfected in a stable permanent way with a variant of the Top-Flash reporter. These cells respond to added purified Wnt3A protein by an increase in luciferase activity. The dynamic range (response to Wnt from 1-200 nanogram/ml Wnt) and increase in luciferase activity (500 fold) is higher than in previously established Wnt reporter systems. The cells are easy to grow and the luciferase response can be automated, making this system suitable for high-through put screens for Wnt inhibitors.

Available material: Cells that stably express a luciferase reporter construct, driven by the TCF transcription factor, including the following cell lines:
  1. Mouse L cells stably expressing a Wnt reporter, responding to Wnt3a protein over a 500 fold dynamic range;
  2. Human 293 cells, stably expressing a Wnt reporter, responding to Wnt3a protein over a 100 fold dynamic range;
  3. Drosophila S2 cells, stably expressing a Wnt reporter and the Frizzled1 receptor, responding to Wingless protein over a 300 fold dynamic range.


Applications


  • To test the activity of wnt signaling in cell culture.

Advantages


  • Quantification and Sensitivity.

Publications



Additional information


A ready-to-sign license agreement for internal use is available here. related docket: S06-500: Wnt proteins to maintain neural stem cells (details) related docket: S05-298: Liposome Mediated Tissue Regeneration (details) related docket: S01-184: Composition and Method to Purify Active Wnt Proteins for Stem Cell Proliferation (details) related docket: S94-013: In Vitro Assay for Wnt growth factor (details)

Innovators & Portfolio



Date Released

 1/11/2011
 

Licensing Contact


Mary Albertson, Senior Licensing Associate
(650) 725-9411 (Direct)
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Related Keywords


wnt signaling   screening: cellular assay   research tool: cell line   screening   research tool   
 

   

  

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