The Office of Technology Licensing was established in 1970 to transfer technologies developed at Stanford. Find out more about OTL's history, mission, staff, and statistics.

Login to TechFinder » 

Customizable, Porous Tissue Engineering Scaffold for 3D Cell Proliferation


Stanford Reference:

12-089


Abstract


Hydrogel-based tissue engineering scaffolds are widely used for culturing cells in three dimensions (3D) due to their tissue-like water content, tunable biochemical and physical properties, and ease of cell encapsulation and distribution in 3D. However, most conventional hydrogels lack the macroporosity desirable for efficient cell proliferation and migration, and have limited flexibility when subject to mechanical load. Microfiber-based scaffolds, on the other hand, are inherently macroporous and flexible, but are often associated with cell-unfriendly fabrication procedures which make microfibers unsuitable for direct cell-encapsulation.

To overcome these limitations, Stanford University Researchers have created a novel scaffold material consisting of crosslinkable, microribbon-like elastomers, which can assemble into highly macroporous scaffolds that facilitate cell encapsulation and support cell proliferation in 3D.


The biochemical composition, macroporosity level, and mechanical properties of the microribbon-based scaffolds can be independently adjusted to influence cell behaviors associated with biochemical, topographical and mechanical cues. Furthermore, the geometry of microribbons enables the microribbon-based scaffolds to sustain large deformation and mechanical stress.


In published results, the researchers showed that, when encapsulated in the microribbon-based scaffold, human adipose derived stromal cells proliferated up to 30-fold within 3 weeks. Furthermore, microribbon-based scaffolds demonstrate great flexibility and can sustain up to 90% strain and 3 MPa stress without failing. Such unique macroporosity and flexibility of the microribbon-based scaffolds make them promising for engineering shock-absorbing tissues such as cartilage and intervertebral discs.


Applications


  • 3D scaffolds of customized macroporosity, biochemical properties, and mechanical properties
    • Implants customized for specific tissue-regeneration (ex: skin, cartilages, bones)
    • A novel research platform for studying cell-responses to different microenvironments of customized biochemical, biophysical, and topographical cues
  • Substrates for 3D cell culture

Advantages


  • Highly Tunable Properties – control over porosity, biochemical and mechanical properties
  • Direct Cell-Encapsulation – enables control over cell distribution
  • Flexibility – can sustain up to 90% strain and return to its original shape
  • This technology enables combination of the benefits of both hydrogel and microfiber-based tissue engineering scaffolds
    • Flexible
    • Macroporous
    • Direct Cell-Encapsulation

Publications



Innovators & Portfolio



Date Released

 4/7/2014
 

Licensing Contact


Grace Yee, Licensing Associate
650-723-8510 (Business)
Login to Request Information

97-042 OptoBondTM - Hydroxide and Silicate Bonding
97-161 Thin Films Having Rock-Salt-Like Structure Deposited on Amorphous Surfaces
99-046 Electroactive Polymers for Opto-Electronic Devices

more technologies

Related Keywords


materials: polymer   materials: composite   therapeutic: wound healing   therapeutic: stem cell   therapeutic: cell signalling   chemical: polymers   advanced materials   biomaterials   cell culture   cell proliferation   medical devices: drug delivery   proliferation   therapeutic: drug delivery   
 

   

  

Also of Interest...
97-042 OptoBondTM - Hydroxide and Silicate Bonding
97-161 Thin Films Having Rock-Salt-Like Structure Deposited on Amorphous Surfaces
99-046 Electroactive Polymers for Opto-Electronic Devices

Recently Viewed...
S12-089 Customizable