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Method of in situ oligonucleotide synthesis on magnetic beads


Stanford Reference:

12-278


Abstract


Researchers at Stanford have developed a method for synthesizing oligonucleotides directly onto super-paramagnetic beads (≤ 1 um). All the reagents required for this approach are readily available from commercial vendors, and the columns and external permanent magnetic spheres are resuable. Since this is an in situ synthesis, beads with oligonucleotides are immediately ready for binding to a primer or other target strands in solution. This is a much more straightforward process than methods that require first a separate oligonucleotide synthesis, then modification with expensive monomers such as thiol or biotin and time consuming removal from the substrate, followed by binding to beads for sequencing. Streptavidin is a challenge in itself with respect to 1) low binding efficiency and 2) free biotin competition with active sites on the bead.



Applications


  • Direct target probe capture and DNA sequencing

Advantages


  • Better than the streptavidin binding approach, which has limitations

Publications



Innovators & Portfolio


  • Michael Akhras   
  • Michael Jensen   

Patent Status



Date Released

 10/8/2013
 

Licensing Contact


Ximena Ares, Licensing Associate
650-724-0960 (Direct)
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Related Keywords


DNA synthesis   DNA sequencing   magnetic beads   next generation sequencing   nucleic acid sequencing   oligonucleotide probe   Oligonucleotide Synthesizer   pyrosequencing   research tool: DNA/RNA sequencing   research tool: DNA/RNA synthesis   research tool: oligonucleotide synthesis   
 

   

  

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S12-278 Method of in situ oligonucleotide synthesis on magnetic beads