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Multiplexed intracellular recording using nanopillar electrodes


Stanford Reference:

13-019


Abstract


Researchers in Dr. Bianxiao Cui’s lab have developed nanopillar electrodes to improve the scalability and reduce the invasiveness of intracellular electrophysiology recording. Accurate measurement of intracellular potentials is critical in understanding electrophysiology properties of excitable cells, such as neurons and cardiomyocytes. It is also important for screening drugs that target ion channels. However, the traditional patch clamp method of intracellular recording suffers from invasiveness, lack of multiplexing and implementation complexity. This technology overcomes these limitations by providing the nanopillar electrodes. After a brief electroporation pulse, the nanopillar electrodes record intracellular action potentials with excellent signal strength and quality. The electrodes provide long-term measurement, high sensitivity and minimal invasiveness.

Stage of Research
The inventors have shown that the vertical nanopillar electrodes can record both the extracellular and intracellular action potentials of cultured cardiomyocytes over a long period of time with excellent signal strength and quality.

Ongoing Research
The next generation of nanoelectrode, nanotubes of iridium oxide, is under development by the inventors and has been shown to further increase the recorded signal amplitude and intracellular access durations.

Applications


  • Basic electrophysiology research
    • Measuring membrane potentials from excitable cells
    • Cell development and differentiation
    • Membrane repair biology
  • Drug development
    • High-throughput screening of drugs that target ion channels

Advantages


  • High-throughput, easily multiplexed recording
  • Minimally invasive
  • Long-term recording- monitor same cell over lifespan of the culture
  • High sensitivity
  • High fidelity
  • Robust for repeated recordings
  • Nanoscale electroporation and resealing allows repeated switching between extracellular and intracellular recording
  • Can be used to mechanically pin down mammalian cells

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Date Released

 2/3/2014
 

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Related Keywords


drug screening   electrodes   electrophysiologic monitoring   electrophysiology   electroporation   patch clamp   cell analysis   cell physiology   cellular analysis   cellular imaging   high-throughput screening   intracellular analysis   preclinical drug screening   ion channel   ion channels   
 

   

  

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