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Dandybodies: DNA-Conjugated Antibodies for Improved Antibody Affinity and Reduced Antibody Cross Reactivity


Stanford Reference:

13-273


Abstract


The sandwich immunoassay is a common strategy used for protein detection and has repeatedly been adapted to various platforms. One example is that of the protein microarray where multiple capture antibodies each targeted against a different antigen are immobilized in a spatially addressable format onto a glass surface. The sample to be analyzed is incubated allowing any antigens present in the sample to bind, and the resulting bound antigens are detected using a pool of detector antibodies that are conjugated to either a fluorophore or enzyme that will effect a colormetric change when reacted with its substrate. While such antibody microarrays offer the ability to assay multiple antigens from a single sample simultaneously, they are inherently limited in sensitivity and throughput by the quality of the antibodies available. Low affinity antibodies result in false negatives due to weak binding, and nonspecific antibodies result in false positives due to cross reactivity with non-specific antigens. In the current state of technology, the upper limit of the number of antigens that reliably can be analyzed simultaneously using an antibody microarray is roughly twenty antigens. Researchers at Stanford have invented a technique using Dandybodies (DNA-conjugated antibodies) that can be adapted to any pre-existing antibody to both improve affinity and reduce cross reactivity. Their technique thus promises to drastically improve the accuracy and throughput of antibody microarrays and other sandwich immunoassay platforms, and in turn facilitate large-scale multiplexed protein analysis.

This invention is closely related to Dockets S09-198, New Functionalized Microfluidic Channels Device and S11-353, Multiplexed Microfluidics Immunoassays Using Dielectrophoresis Elution.

Applications


  • Multiplexed protein detection (allows for multiplexed protein detection greater than 20 analytes in parallel)
  • Improving antibody affinities
  • Reducing cross reactivity in antibody arrays

Advantages


  • Universally adaptable to any pre-existing antibody

Publications



Innovators & Portfolio



Date Released

 9/6/2013
 

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Related Keywords


research tool: antibody   research tool: hybridization   diagnostic: antibody   diagnostic: biochip   diagnostic: detection   diagnostic: DNA probe   diagnostic: elisa   diagnostic: immunoassay   diagnostic: point-of-care   Genome Center   13-273   
 

   

  

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S13-273 Dandybodies: DNA-Conjugated Antibodies for Improved Antibody Affinity and Reduced Antibody Cross Reactivity