Activity-dependent expression system for labeling and optogenetic control of neuronal circuits

Researchers in Prof. Karl Deisseroth's laboratory have engineered versatile, virus-based constructs that are driven by neuronal activity to either label or optogenetically control those active neurons. These vectors can be administered to any brain region where they function to express desired proteins in neurons that are active during distinct experiences of freely moving animals. The expression system utilizes a minimal c-fos-based promoter packaged into AAV particles and designed to specifically drive protein synthesis in active neurons in a variety of mammalian species. Downstream, the time-locked activity is translated into sustained gene expression, which can then facilitate either imaging of complex cell activity or optogenetic control of cell responses.

Stage of Research
The inventors used this tool in mice to visualize wiring (axonal projections) and molecular features in the prefrontal cortex associated with distinct behavioral experiences. They identified brain-wide projection patterns and discovered unique and unexpected cell responses.