Anti-Pbx (long form) monoclonal antibody

Researchers in the laboratory of Dr. Michael Cleary at Stanford University have developed anti-Pbx (long form) monoclonal antibodies to study transcriptional regulation, embryonic development, and tissue homeostasis. Pbx genes code for a family of highly conserved homeodomain proteins of the TALE (three-amino acid loop extension) class that participate in multiprotein complexes to regulate developmental gene expression. Pbx proteins also heterodimerze with the Meis/Prep subfamily of TALE-class homeodomian proteins to form trimeric complexes with Hox proteins on appropriate DNA sites that regulate developmental gene expression. Additional isoforms of mammalian Pbx proteins arise from differential splicing of Pbx transcripts to yield high molecular-weight (MW) (Pbx1a, Pbx2, Pbx3a, and Pbx4) and low-MW (Pbx1b and Pbx3b) forms of the respective proteins. The anti-Pbx (long form) monoclonal antibody recognizes three Pbx high-MW isoforms.