The Nolan laboratory has created second-generation retrovirus producer lines, termed Phoenix, for the generation of helper free ecotropic and amphotropic retroviruses.
The FELIX vector system, like the PHOENIX MLV-based packaging system, produces high-titre retroviral particles capable of stably transducing a wide variety of target cells with a gene of interest.
293T is a human cell line that has unique properties useful in many applications, including those requiring efficient transfection, gene expression, DNA replication, or viral production.