A team of Stanford researchers have developed a simple, novel, non-viral technique for generating human induced pluripotent stem cells (hiPSCs) with minicircle DNA. This technology uses a single minicircle vector that expresses four reprogramming factors.
Rat monoclonal antibody BZ194 specifically recognizes mouse serpentine receptor mCMKLR1 (aka ChemR23, DEZ). mCMKLR1 is a novel protein possessing high homology with members of the chemoattractant receptor family, and binds the chemoattractant chemerin.
This mouse model of phosphodiesterase deficiency was developed using homologous recombination to knock-out the gene for PDE4D. The mice have a null PDE4D gene on C57BL/6 x 129/OLA background. These mice have proven useful in studies of asthma (see publications).
This mouse model of phosphodiesterase deficiency was developed using homologous recombination to knock-out the gene for PDE4B. The mice have a null PDE4B gene on C57BL/6 x 129/OLA background.
There are two aspects to this invention, the RetroTet-ART vectors themselves, and the use of those vectors to identify novel regulatory elements (untranslated regions, or UTR's).