Stanford researchers at the Swartz Research Group have engineered an Iron-Iron (Fe-Fe) hydrogenase with as high as 5-fold enhancement in O2 tolerance by introducing cysteine mutations around the electron supply pathway within the enzyme.
Enzymatically active hydrogenase is synthesized in a cell-free reaction. The hydrogenases are synthesized in a cell-free reaction comprising a cell extract derived from microbial strains expressing at least one hydrogenase accessory protein.