Mouse Ab against the C terminal Fragment of Fly Patched

Hybridoma cells for the production of monoclonal antibodies against C-terminal fragment of fly patched were made by fusion of spleen cells from an immune mouse and sp2/0 myeloma cells. Positive clones were detected by Western blotting and immunocytochemical staining of Schneider cells. After limited dilution subcloning several positive subclones were obtained.