Composition and Method to Purify Active Wnt Proteins for Stem Cell Proliferation

Applications

• Biochemical characterization of Wnt signaling
• Maintain and expand stem cell populations for research purposes
• Potential broad applications of use of the purified Wnt proteins in fields such as transplantation and tissue regeneration

Advantages

• First effective method to purify Wnt proteins - full purification never done before
• First time useful amounts of pure, biologically active Wnt proteins available
• Allows maintanence of stem cell cultures - not possible before
• Allows for more effective transplantation and tissue regeneration

Publications

• Reya T et al. A role for Wnt signalling in self-renewal of haematopoietic stem cells. Nature. 2003 May 22;423(6938):409-14.
• Willert K et al. Wnt proteins are lipid-modified and can act as stem cell growth factors. Nature. 2003 May 22;423(6938):448-52.
• US patent 7,153,832: Compositions of active Wnt protein
• US patent 7,335,643: Compositions of active Wnt protein
• US patent application 12/480,550: Compositions of active Wnt protein
• related publication: Couzin J. Purified Signaling Protein Stimulates Stem Cell Proliferation. Science. 2003 May 2;300(5620):722.

Wnt Gene Homepage

the Wnt homepage

Patents

• Published Application: WO2004091647
• Published Application: 20040248803
• Published Application: 20070105776
• Published Application: 20070269890
• Published Application: 20090275134
• Published Application: 20150344846
• Published Application: 20160097031
• Published Application: 20180002661
• Issued: 7,335,643 (USA)
• Issued: 8,642,335 (USA)
• Issued: 9,139,636 (USA)
• Issued: 10,066,206 (USA)