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Anti HA Monoclonal Antibody


Stanford Reference:

02-058


Abstract


Rat monoclonal antibody isotype IgG2a (clone #153) recognizes the HA peptide sequence [YPYDVPDYA] derived from the influenza hemagglutinin protein. The HA peptide can be added to unrelated proteins through recombinant techniques. The resulting "epitope-tagged" fusion protein can be detected using the anti-HA monoclonal antibody #153. Through the use of epitope-tagged proteins, scientists can use the anti-HA monoclonal antibody to help determine the function of proteins encoded by uncharacterized genes. For example, a HA epitope can be aded genetically to a newly discovered gene, and that gene can be expressed in a cell line. Cells expressing the gene can be selected for and the function of the encoded protein determined by various assays.Scientists have used epitope -tagged proteins to determine:
the size, cellular location, and post-transitional modifications of newly discovered genes;
the trafficking of proteins within cells;
the identitu of other proteins interacting with tagged proteins.

The antibody can also be used in immunoblotting (i.e. Western blots), immunoprecipitation, immunoassays (i.e. ELISA), immunocytochemistry, immunfluorescence, and flow cytometry.

Applications


  • Immunoblotting
  • Immunoprecipitation
  • Immunoassays
  • Immunocytochemistry
  • Immunofluorescence
  • Flow cytometry

Advantages


  • Rat anti-HA monoclonals are extremely useful in mouse systems--epitope-tagged cells transferred into mice can easily be identified by using the rat anti-HA monclonal antibody.

Innovators & Portfolio



Date Released

 3/14/2002
 

Licensing Contact


Brenda Martino, Biological Materials Specialist
(650) 725-9119 (Direct)
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Related Keywords


research tool   diagnostic: antibody   diagnostic: immunoassay   research tool: antibody   research tool: cell line   
 

   

  

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78-017 Monoclonal Antibodies: 11-6 anti-Ig-5b, 11-5 anti-I-A (2), 11-4 anti-H-2Kk, 11-3 anti-I-A917), 10-4 anti-Ig-5a, H-12 anti-THY 1.2
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S02-058 Anti HA Monoclonal Antibody