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High-throughput barcoding method for highly accurate single molecule profiling


Stanford Reference:

13-425


Abstract


Researchers at Stanford have developed a high-throughput barcoding method that greatly improves sequencing accuracy and makes it possible to do robust single molecule profiling, since it can trace duplicate sequencing reads to their original single molecule clones. Characterization of genetic information in biological samples requires both accurate quantification and characterization of variants. Recently, molecular barcodes have been developed to improve gene expression measures, especially for low input amounts and for single cell transcriptome analysis. However, current barcoding methods either do not scale up well or are prone to sequencing errors that disrupt analysis. To overcome these limitations, the inventors have developed exponentially-expanded barcodes (EXBs). EXBs use a computationally designed set of highly error-resistant barcodes followed by a combinatorial enzymatic assembly process to generate high diversity without sacrificing robustness. This method generates exponentially large numbers of unique, rationally-designed barcodes that can be used to accurately quantify each molecular species present in the sequencing reaction.

Stage of Research
To assess the method’s performance, the researchers have applied it to known gene expression standards at varying input amounts, and to the analysis of single cells. They found EXBs are superior to other barcoding methods, since they enable molecular tagging that is resistant to errors for both bulk and single cell transcriptomes.

30 Minute Webinar



On January 24, 2018, Billy Lau, co-inventor of the technology, gave an overview of the technology, including potential applications and competitive differentiation.



Applications


  • Analysis of sequencing results:
    • Single molecule profiling and rare mutation confirmation
    • Quantification of molecular species
    • Distinguishing between PCR duplicates and distinct molecules in amplicon or deep resequencing
    • Confirmation of mutations at low sequencing depth

Advantages


  • Highly scalable (~10"10 and higher)
  • Easily modified to larger and smaller barcodes sizes
  • Robust and systematic generation of barcodes
  • Robust mapping to designed barcode
  • Resistant to PCR and sequencing errors
  • Applicable to bulk and single cell transcriptomes

Publications



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Innovators & Portfolio



Date Released

 10/12/2017
 

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Ximena Ares, Senior Licensing Associate
650-724-0960 (Direct)
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Related Keywords


gene sequencing   research tool: DNA/RNA sequencing   genomic analysis   low-coverage sequencing   genotyping   next generation sequencing (NGS)   resequencing   nucleic acid sequencing   dna sequencing   deep sequencing   3GS   diagnostic: sequencing   Single molecule sequencing   RNA sequencing   sequencing technology   
 

   

  

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S13-425 High-throughput barcoding method for highly accurate single molecule profiling