CRISPR/Cas9 Knock-in Mice

Researchers in Prof. Monte Winslow's laboratory have developed two viable, fertile transgenic mouse strains that enable rapid, simple generation of loss-of-function models with CRISPR/Cas9 mediated genome editing in vivo or ex vivo. These mice can be used to inactivate genes of interest without the time and expense needed for traditional knock out mice. Specifically, the H11LSL-Cas9 knock-in mice have Cre recombinase-dependent expression of Cas9 directed by a CAG promoter. There is an additional strain without the LSL cassette that can be used for genome editing with vectors that do not carry Cre. These mice enable the rapid functional investigation of any gene of interest and make loss-of-function experiments in vivo no more difficult than altering those genes in vitro.

Jackson Labs Data Sheets
Stock No: 027650
Stock No: 026816

Stage of Research
The inventors have used this mouse strain to study pancreatic cancer by initiating pancreatic neoplasias that progress into invasive and metastatic tumors. They continue to use these strains to generate additional mouse models of human cancer.