Method for Generating Endothelial Cells from Pluripotent Stem Cells

Stanford researchers have developed a novel xeno-free, no purification, robust, quick, low-cost method for generating pure endothelial cell cultures by sequentially inducing mesodermal-lineage cells from pluripotent stem cells, using differentiation stage-specific extracellular signals. The process, conducted in a xeno-free condition, involves culturing mesoderm-lineage cells in an endothelial progenitor cell specification medium, then switching to a complete endothelial cell growth medium for maturation and expansion. Additionally, methods for screening optimal extracellular signal cocktails are provided for each differentiation stage. These methods allow for the mass production of high-purity vascular endothelial cells in large quantities, within a short time frame.

Stage of Development
In vitro