Multiplexed Bead-Based Assay for High-Throughput Biomolecule Detection

Stanford researchers have developed a next-generation bead-based assay platform that enables highly scalable, multiplexed detection of proteins, antibodies and oligonucleotides in a single run.

Traditional biomolecule detection methods, such as ELISA and fluorescent bead-based assays, are slow, labor-intensive, and limited in scalability and multiplexing. They struggle to analyze multiple targets simultaneously, restricting their efficiency in high-throughput applications like large-scale studies, diagnostics, and drug discovery.

To address these limitations, Stanford researchers have developed a next-generation bead-based assay platform that enables highly scalable, multiplexed detection of proteins, antibodies and oligonucleotides in a single run. This system utilizes isotope-barcoded single-bead technology and an ICP-MS-TOF instrument, allowing for the simultaneous analysis of multiple targets per bead across thousands of samples. The system is designed for flexibility, and it can be configured for antibody detection, protein quantification, oligonucleotide analysis, or functional readouts. Collectively, this scalable and flexible system is a powerful tool for biomarker discovery, drug development, and diagnostics.

Stage of Development:
Proof of Concept