Multipotent Neural Stem Cells Derived From Human Embryonic Stem Cells

Stanford scientists have successfully developed the first method for isolating normal, homogeneous, expandable and multipotent neural stem-progenitor cells (NSPCs) from human embryonic stem cells (hESCs), using a defined in vitro method of selection and perpetuation based on their intrinsic properties. The isolated NSPCs demonstrate an unlimited self-renewable capacity with normal characteristics, stable growth rate, differentiation profile and consistent generation of the three principal CNS cell types (neurons, astrocytes and oligodendrocytes), thereby eliminating the need for a lineage-specific treatment. The NSPCs are perpetuated using a single cell dissociation protocol that is critical for the accurate measurement of cell viability, growth rate and potency assays. Consequently, this process qualifies for large-scale production under the current good manufacturing practices and for a reliable quality assurance program for potential use of these cells in a clinical setting.

An experiment with a stroke rat model has demonstrated the potential for these NSPCs as a cellular therapy. The grafted NSPCs readily integrate and differentiate within the damaged area in a rat brain, without overgrowth or tumor formation. Thus, these NSPCs appear well suited for therapy of a range of neurological disorders.