Super Recombinator (SuRe), a CRISPR/Cas9-based platform for the rapid incorporation of multiple transgenes at the same genetic locus

Incorporation of genetic elements from foreign species to generate transgenic organisms in bacteria, fungi, plants, and animals for a variety of applications ranging from food crops, industrial enzymes, basic research, etc., has transformed our life. To achieve complex functions for academic research, agriculturial production, or industrial production, it is desirable to create transgenic organisms with multiple transgenic elements. Because of the underlying constraints imposed by genetic linkage and crossing-over, the incorporation of multiple transgenes is difficult, time-consuming, and largely limited to one transgene. Harnessing the recent advancement in CRISPR/Cas9 technology, researchers at Stanford created a novel system termed Super Recombinator (SuRe) that enables a significant reduction in turnover time for introducing N transgenes in the same locus from N-1 to log₂N steps. Moreover, this strategy requires the selection of much fewer possible genotypes per step and does not involve embryo injection, thus further reducing labor, cost, and time. The success of the strategy has been demonstrated in vivo.

Stage of Development
In vivo demonstration of successful incorporation of multiple transgenes in animal brain.