An in vivo reporter of proteasome function

The invention consists of a plasmid encoding enhanced green fluorescent
protein (GFP) modified with a short targeting sequence appended to its
carboxyterminus. This targeting sequence converts the normally stable
GFP molecule into a short-lived protein and substrate of the ubiquitin
proteasome pathway. A clonal cell line, GFPu-1, expressing this
contstruct has been isolated. The fluorescence level of GFPu-1 cells is
proportional to the activity of the ubiquitin proteasome system.

Available from ATCC CRL-2794 for evaluation/non-commercial use.

Reference:
Science, Vol.292, pp1552-1555, 2001.