Increasing the size of rAAV-mediated expression cassettes in vivo

Researchers at Stanford have developed and patented rAAV vectors that can be used to transfer long nucleic acids into cells. Recombinant AAV vectors are limited by a restricted amount of exogenous DNA that can be placed into the vector. The strategy designed here overcomes this problem by using two different rAAV vectors, each containing a portion of an expression cassette to reconstitute gene expression in vivo. By strategic placement of cis DNA elements, up to 70% of the exogenous level of gene expression from a single vector can be achieved in vivo.

Please see the issued patent (below) for additional information.