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Fluorescent Calcium Biosensor to track dynamic calcium signaling in live cells


Stanford Reference:

05-146


Abstract


Researchers in Prof. Tobias Meyer’s laboratory have developed a patented, simple, rapid and robust assay to track dynamic calcium signaling in live cells using a fluorescent Calcium Biosensor Peptide (CBP). Calcium signaling in cells is an important mechanism to regulate immune function, with implications for autoimmune diseases, immunodeficiencies, asthma and other conditions. Compared to conventional methods to track intracellular calcium, such as patch-clamping or pre-loading dyes, this CBP-based technology is less labor-intensive, scalable, and more accurate. Cells expressing the CBP can be used in basic research of the calcium signaling process or to screen for agents that influence this cellular activity, particularly for agents that act on calcium channels such as ORAI proteins.

Applications


  • Drug screening - identifying therapeutic agents (gene products, small molecules, or environmental stimuli) that modulate intracellular calcium for treatment of autoimmune diseases, immunodeficiencies, asthma, cancer (immunotherapy) and other conditions
  • Research - studies of calcium signaling mechanisms downstream of GPCR and other receptors

Advantages


  • Simple, rapid and robust assay
  • Scalable - can be adapted for high-throughput screening
  • Stable - CBP is expressed in the cell, eliminating the need for pre-loading with dye
  • Accurate
    • fluorescent marker, unlike dyes, does not interfere with calcium buffering in cell
    • an internal control is generated for accurate measurement
  • Non-toxic - fluorescent marker, unlike dyes, are not toxic to cells
  • Flexible time for interpretation - assay can be read in real time or fixed for later analysis
  • Dynamic and reversible - can be used in tracking dynamic calcium and reversible calcium signaling events in live cells during physiological processes

Publications



Web Site


Meyer Lab

Innovators & Portfolio



Patent Status



Date Released

 9/11/2012
 

Licensing Contact


Ximena Ares, Licensing Associate
650-724-0960 (Direct)
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Related Keywords


calcium   ion channel biology   ion channel   cell signaling   protein folding   immunological signaling   immunology   calcium channel   
 

   

  

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