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Oligonucleotide Synthesis


Stanford Reference:

06-082


Abstract


Researchers at the Stanford Genome Technology Center have developed a patented, inexpensive, robust method of fabricating long synthetic oligonucleotides for molecular inversion probes, padlock probes, and proximity-ligation assays. This technology is based on long padlock probes that capture and amplify up to 500 bases of DNA. The technique does not rely on chemical synthesis, thereby eliminating the size constraint of the single-stranded product. In addition, the production of the molecules does not rely on the sequence of the desired strand. This method was demonstrated in a multiplex PCR format, and can be applied to a variety of DNA templates, including plasmid, viral, genomic DNA or synthetic DNA.

Applications


  • Oligonucleotide synthesis - generation of large single-stranded DNA molecules from a variety of templates, with end user applications in:
    • Molecular Inversion Probes
    • Padlock Probes
    • Proximity Ligation Assays
    • Sequencing
    • Clinical sequencing for gene panels

Advantages


  • Robust - probes generated with this technique are able to function robustly by extending over larger distances than the conventional-sized molecular inversion probes
  • Inexpensive - does not require chemical synthesis of DNA
  • Simple - does not require chemical modification of primers
  • Multiplex ability
  • No size constraints

Publications



Web Site


Stanford Genome Technology Center

Innovators & Portfolio



Patent Status



Date Released

 4/13/2015
 

Licensing Contact


Ximena Ares, Licensing Associate
650-724-0960 (Direct)
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Related Keywords


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