Light sheet fluorescence microscopy using high speed structured and pivoting illumination

Researchers at the Solgaard Lab have demonstrated that light sheet fluorescence microscopy (LSFM) with structured and pivoting illumination enables fast image acquisition and improved image quality. A one-dimensional spatial light phase modulator, grating light valve (GLV), is used to control the illumination profile at high speed (up to several hundred kHz) in LSFM. Structured and pivoting illumination can be switched without any optical or mechanical adjustments providing a more stable measurement.
To demonstrate the features of the system, the researchers imaged fluorescent beads and biological samples, successfully obtaining optically sectioned images with higher contrast using structured illumination and with reduced shadowing effects using pivoting illumination.

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Stage of Research