Docket #: S01-135
An in vivo reporter of proteasome function - ATCC CRL-2794
The invention consists of a plasmid encoding enhanced green fluorescent
protein (GFP) modified with a short targeting sequence appended to its
carboxyterminus. This targeting sequence converts the normally stable
GFP molecule into a short-lived protein and substrate of the ubiquitin
proteasome pathway. A clonal cell line, GFPu-1, expressing this
contstruct has been isolated. The fluorescence level of GFPu-1 cells is
proportional to the activity of the ubiquitin proteasome system.
Available from ATCC CRL-2794 for evaluation/non-commercial use.
Reference:
Science, Vol.292, pp1552-1555, 2001.
Applications
- To measure intracellular activities and flux throught the
- ubiquitin-proteasome pathway
Advantages
- Other known reporter system use different targeting sequences that are
- not as general as the one used here
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