This mouse model of phosphodiesterase deficiency was developed using homologous recombination to knock-out the gene for PDE4D. The mice have a null PDE4D gene on C57BL/6 x 129/OLA background. These mice have proven useful in studies of asthma (see publications).
This mouse model of phosphodiesterase deficiency was developed using homologous recombination to knock-out the gene for PDE4B. The mice have a null PDE4B gene on C57BL/6 x 129/OLA background.
There are two aspects to this invention, the RetroTet-ART vectors themselves, and the use of those vectors to identify novel regulatory elements (untranslated regions, or UTR's).