Mutated Renilla Luciferase For Higher Light Output and Altered Stability

Stanford researchers have developed mutant Renilla luciferase proteins and reporter gene constructs which modify the physical characteristics of the Renilla luciferase protein for use in biological assays. The mutant Renilla Luciferases contain specific mutations which confer altered protein stability and enhanced light output. For reporter gene applications, a mutant can be choosen with the appropriate level of stability for either transient or constitutive applications. The stabilized variants have potential for use as bioluminescent tags for both in vivo and in vitro applications.

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Figure description - RLuc7-521 experimental validation

Stage of Research
Validated red-shifted variant of RLuc (RLuc7-521) for transient reporter gene imaging