S13-492 3D Super Resolution Microscopy with a Bisected Phase Mask: A Device to Measure the Depth, Position and Orientation of Single Molecules Stanford researchers have designed a tunable wedge-based phase mask for 3D super-resolution imaging that can simultaneously determine both the position and rotational mobility of individual light-emitting molecules from a single camera image. Adam Backer Mikael Backlund Alexander Diezmann Steffen Sahl