293T is a human cell line that has unique properties useful in many applications, including those requiring efficient transfection, gene expression, DNA replication, or viral production.
The Nolan laboratory has created second-generation retrovirus producer lines, termed Phoenix, for the generation of helper free ecotropic and amphotropic retroviruses.
Rapid, Stable, High titre production of Recombinant Retrovirus
This method of rapid production of high titre retrovirus for large scale production involves a retrovirus construct with an Epstein Barr Virus origin of replication and EBNA protein.
The FELIX vector system, like the PHOENIX MLV-based packaging system, produces high-titre retroviral particles capable of stably transducing a wide variety of target cells with a gene of interest.
Stanford researchers have developed an improved method of distinguishing live and dead cells using mass cytometry, a next-generation form of flow cytometry.
Stanford scientists developed a new chemistry technique involving embedding tissue in hydrogel for mass spectrometry (MS), without the need for water to prepare samples for MS, that provides better resolution, better maintenance of X-Y distances and subsequent improvement in v
Stanford researchers have developed a versatile computational approach for easily visualizing and analyzing multidimensional molecular data, such as flow cytometry data.
A team of Stanford researchers has identified mutations in the LNK gene in a subset of patients with chronic myeloproliferative neoplasms (MPNs). LNK (also known as SH2B3) is an adaptor protein that inhibits JAK-STAT signaling.
Researchers at Stanford have developed a method to direct T cell fate toward the T stem cell memory (TSCM) phenotype during ex vivo expansion for adoptive cell transfer (ACT) therapies.
Stanford researchers have created a technology using CyTOF (Cytometry by Time Of Flight mass spectrometry) and CODEX (CO-Detection by indEXing) imaging to systematically analyze cell therapies produced ex vivo and their effects in vivo.
Compressed sensing has revolutionized signal acquisition by enabling high dimensional signals to be measured with remarkable fidelity using a small number of so-called incoherent sensors.